Nico Rosberg upped the pace on Tuesday morning after bolting on a set of soft tyres to post a 1:23.022.
The German's best was just two-tenths shy of Sebastian Vettel's best laptime from last week's test, despite the Ferrari man using Pirelli's new quicker Ultra-Soft compound.
Rosberg also topped the mileage counter, lapping the Circuit de Barcelona-Catalunya 82 times before lunch, after which he will hand his car over to team-mate Lewis Hamilton.
Kimi Raikkonen was second fastest ahead of McLaren's Fernando Alonso who enjoyed a far better morning compared his final run last week which saw him complete just three laps. The Spaniard completed 50 laps before stopping at the pitlane exit to cause a red flag.
Felipe Nasr debuted the new Sauber C35 for the first time on-track, posting a 1:26.923 to go ninth fastest before lunch.
Manor's Rio Haryanto completed just one lap before he was sidelined with an oil leak according to his team.
Pre-season testing – Test 2 – Day 1 Morning Times:
| Pos | Driver | Team | Time | Gap | Tyre | Laps |
| 1 | N. Rosberg | Mercedes | 1:23.022 | 0.578 |
 |
82 |
| 2 | K. Raikkonen | Ferrari | 1:24.836 | 1.814 |
 |
42 |
| 3 | F. Alonso | McLaren | 1:25.252 | 2.230 |
|
50 |
| 4 | V. Bottas | Williams | 1:25.255 | 2.233 |
|
58 |
| 5 | M. Verstappen | Toro Rosso | 1:25.356 | 2.334 |
|
59 |
| 6 | N. Hulkenberg | Force India | 1:25.954 | 2.932 |
|
50 |
| 7 | D. Kvyat | Red Bull | 1:26.227 | 3.205 |
|
22 |
| 8 | E. Gutierrez | Haas | 1:26.661 | 3.639 |
|
23 |
| 9 | F. Nasr | Sauber | 1:26.923 | 3.901 |
|
42 |
| 10 | K. Magnussen | Renault | 1:26.981 | 3.959 |
|
54 |
| 11 | R. Haryanto | Manor |
 |
1 |
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