Tech3 KTM rider Maverick Vinales topped the charts for the second time, leading the field at the conclusion of the final MotoGP practice at Mugello.
The Spaniard waited till the end of the session to post his lap, recording a time of 1.45.742s.
Factory Ducati rider Marc Marquez led throughout the vast majority of the session, but lapped 0.016s slowly at the end of the 30-minute session.
Fabio Quartararo pulled off an impressive third for factory Yamaha, finishing ahead of title contender Alex Marquez.
KTM’s rise in competitiveness this weekend continued as 21-year-old Pedro Acosta finished fifth for the factory outfit.
Fabio Di Giannantonio and Franco Morbidelli slotted their VR46 Ducati’s in sixth and eighth, respectively, with Aprilia’s Marco Bezzecchi sandwiched between.
Fermin Aldeguer improved on Friday to finish in ninth, with three-time Mugello race winner Francesco Bagnaia down in 10th place.
Enea Bastianini secured 11th for Tech3 KTM, while Honda duo Joan Mir and Takakki Nakagami made a step overnight.
Brad Binder claimed a surprise 14th, but posted a lap quicker than Trackhouse’s Ai Ogura and Raul Fernandz in the closing minutes.
Miguel Oliveira finished in 17th ahead of Johann Zarco, before Yamaha riders Alex Rins and Jack Miller occupying 19th and 20th.
Lorenzo Savadori and Somkiat Chantra once again took up the last two places.